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Research PeptidesCAS 53-84-91000 mg per vial

NAD+ Research

Mechanism, pharmacology, published literature, reconstitution and stability for β-nicotinamide adenine dinucleotide, free acid.

NAD+ research vial

NAD+ 1000 mg is supplied by Healthy Peps as a high-purity nicotinamide adenine dinucleotide preparation for in vitro redox and enzymology research, shipped from Canada with batch documentation.

HP

Healthy Peps Research Team · Research Editorial Team

Last reviewed November 2026. For in vitro laboratory research only.

Physicochemical Reference Data

Compoundβ-Nicotinamide adenine dinucleotide, free acid
CAS Number53-84-9
Molecular FormulaC21H27N7O14P2
Molecular Weight663.43 g/mol
AppearanceWhite to off-white powder
Solubility (lab)Highly soluble in water; buffer-compatible
Vial Format1000 mg per vial
Storage−20 °C, desiccated, protected from light

Mechanism & Research Context

NAD+ is the central redox coenzyme of cellular metabolism, cycling between oxidised NAD+ and reduced NADH in glycolysis, the TCA cycle and oxidative phosphorylation. In the research literature it is also the substrate for two enzyme families of intense interest: the sirtuins and the PARPs, both of which consume NAD+ as part of their catalytic cycle.

That second role is why NAD+ appears so often in ageing and metabolic research: published work describes declining tissue NAD+ with age and examines whether restoring NAD+ availability alters sirtuin activity, mitochondrial function or DNA-repair signalling in cell and animal models. Enzyme-kinetics studies use defined NAD+ concentrations as the rate-limiting substrate for dehydrogenase assays, which is why a precise, documented quantity matters more here than in almost any other catalogue item.

The 1000 mg format suits laboratories that prepare their own working stocks and need a substantial, single-batch quantity rather than many small vials. Assay designers typically prepare fresh NAD+ solutions because the coenzyme hydrolyses slowly in aqueous solution, particularly at neutral or alkaline pH.

Healthy Peps supplies NAD+ as a free-acid powder with a documented purity result and batch number.

How It Compares to Related Peptides

Unlike the peptide vials on this catalogue, NAD+ is a small-molecule coenzyme: it is water-soluble at high concentration, is not reconstituted from lyophilized cake, and is used as an assay substrate rather than a receptor ligand. It also pairs with NADH precursor research compounds in metabolic studies.

Reconstitution, Stability & Handling

Prepare working solutions fresh in water or the buffer specified by your assay. NAD+ hydrolyses in aqueous solution, so solutions should be kept cold and used promptly rather than stored for extended periods. The bulk powder should be kept desiccated at −20 °C, protected from light, and allowed to equilibrate to room temperature before opening to avoid condensation.

Selected Reading & Research Notes

  • NAD+ and sirtuin biology literature. Reviews describing NAD+ as the rate-limiting substrate for sirtuin deacetylation and its decline with age in tissue models.
  • NAD+ / PARP enzymology. Establishes NAD+ consumption by poly(ADP-ribose) polymerases during DNA-damage response assays.

Frequently Asked Questions

Where can I buy NAD+ in Canada?

Healthy Peps ships NAD+ 1000 mg Canada-wide from within Canada, with batch documentation included.

What purity is the NAD+ supplied at?

Consult a published report matching the lot for analytical results. Catalogue specifications alone do not establish the result for a particular batch.

Is NAD+ suitable for human consumption?

No. It is supplied strictly as a laboratory research reagent and is not intended for human or veterinary use.

How should NAD+ be stored?

Keep the sealed powder desiccated at −20 °C, protected from light. Prepare working solutions fresh, as aqueous NAD+ hydrolyses over time.

Why is the 1000 mg format preferable?

A single large, documented batch lets a laboratory prepare many working stocks with consistent purity, instead of re-verifying numerous small vials.

Ready to order NAD+ 1000 mg?

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Related peptide research

All compounds are supplied strictly for in vitro laboratory research and are not intended for human or veterinary consumption. Literature references are reported as published research context, not as established therapeutic claims.