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Peptide Reconstitution Calculator

A laboratory reference tool for reconstituting lyophilized research peptides. For in vitro research use only.

Concentration

Peptide mass ÷ diluent volume = working concentration.

Enter mass and volume to compute concentration.

Dilution from stock

Volume of diluent to add per 1 mL of stock to reach a target concentration.

Enter a stock and a lower target concentration to compute the dilution.

Reconstitution steps

  1. Swab the rubber septum of the lyophilized peptide vial with an alcohol wipe.
  2. Draw the calculated volume of bacteriostatic water into a sterile syringe.
  3. Insert the needle through the septum and direct the diluent down the inside wall of the vial.
  4. Swirl gently to dissolve — do not vortex or shake vigorously.
  5. Confirm the solution is clear and free of undissolved material before use.
  6. Store reconstituted material refrigerated and use within your protocol window; keep lyophilized vials at −20 °C.

Link to this page

A free lab reference your readers will actually use. Copy the snippet below to link to it from your blog, resource list or newsletter.

<a href="https://healthypeps.co/tools/reconstitution-calculator" target="_blank" rel="noopener">Healthy Peps — Peptide Reconstitution Calculator</a>

This calculator is a laboratory reference tool only. All products are sold strictly for in vitro laboratory research and are not intended for human or veterinary consumption.